Table 2.

Frequency of bud formation and cell separation

Relevant genotype	Growth conditions	Bud formation	Cell separation
Wild type	No Lat A	2/2  (100%)	8/8  (100%)
Wild type	Lat A	0/8  (0%)	0/14  (0%)
bee1Δ	No Lat A	17/19  (89%)	15/17  (88%)
bee1Δ	Lat A	0/3  (0%)	0/9  (0%)
Wild type	Galactose	1/3  (33%)	5/13  (38%)
MYO2DN	Galactose	0/13  (0%)	4/37  (11%)

Bud formation was scored in cells without a bud, and cell separation was scored in budded cells. In both cases, cells were observed for 2 h. Lat A, latrunculin A.