Table 1.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pJB254 | YEpHSF1–390 LEU2 | Kopczynski et al., 1992 |
| pJB398 | YEpHSF LEU2 | EcoRI–XhoI fragment from YEpHSF LEu (Torres and Bonner, 1995) transferred to YEplac181 (Gietz and Sugino, 1988), with BglII and KpnI engineered into codons 168 and 440, respectively |
| pJB330 | YEpHSF1–583 LEU2 | Bonner et al., 1994 |
| pJB490 | YCpHSF LEU2 | EcoRI–PstI fragment from pJB398 transferred into YCplac111 (Gietz and Sugino, 1988) |
| pJB491 | YEpSSB1 LEU2 | SSB1 gene from pYW13 (E. A. Craig) cloned into YEplac181 (Gietz and Sugino, 1988) |
| pZ4G | YEpHSE-lacZ URA3 | Synthetic HSE inserted into the XhoI site of pLGΔ178 (Guarente and Mason, 1983; Bonner et al., 1994) |
| pLGΔ312 | YEp-CYC1-lacZ URA3 | CYC1 promoter driving lacZ (Guarente and Mason, 1983) |