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. 2000 May;11(5):1753–1764. doi: 10.1091/mbc.11.5.1753

Figure 4.

Figure 4

Complex III is induced by superoxide or heat. (A) DNA-binding reactions with whole-cell extract to which was added yeast factor or 10 mM 5G2^3. (B) DNA-binding reactions using purified HSF treated with CuPh, with the addition of 20 mM DMSO, ethanol, or 2-propanol (2-Prop) as ·OH scavengers. (C) DNA-binding reactions using purified HSF treated with 10 mM MgCl2 or H2O2 at the indicated concentrations. (D) DNA-binding reactions using purified HSF treated with Fenton reagent (H2O2 + FeSO4) or with H2O2 or FeSO4 alone. (E) DNA-binding reactions using purified HSF treated with CuPh with or without the addition of human erythrocyte superoxide dismutase (SOD). (F) DNA-binding reactions using purified HSF treated with 5 mM MgCl2 with or without the addition of human erythrocyte SOD. (G) DNA-binding reactions using purified HSF incubated at the indicated temperatures.