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. 2003 Mar;71(3):1170–1178. doi: 10.1128/IAI.71.3.1170-1178.2003

FIG. 2.

FIG. 2.

DNA-binding activity of P. gingivalis Dps. Recombinant P. gingivalis Dps purified from the E. coli overexpressing P. gingivalis dps or the recombinant P. gingivalis Dps treated with ferrous ammonium sulfate was incubated with linear DNA (1-kb DNA ladder) at 4°C for 1 h. The mixture was then subjected to agarose gel electrophoresis. DNA on the gel was stained with ethidium bromide. Lanes: 1, DNA (500 ng) alone; 2, recombinant Dps (10 μg) alone; 3, recombinant Dps (10 μg) and DNA (500 ng); 4, iron-loaded recombinant Dps (10 μg) and DNA (500 ng); 5, iron-loaded recombinant Dps (10 μg) alone.