Figure 2.

Time-lapse images of control manipulation performed on metaphase cells. The membrane was pushed perpendicular to the cell axis near the metaphase plate (B), causing no change in the orientation of the long axis of the cell. No change in the position or orientation of the spindle took place, and both anaphase and cytokinesis progressed normally (B–E). Time in minutes relative to metaphase is shown in the upper right corner. Asterisks and dotted lines indicate initial positions of the spindle poles and axis, respectively. Circles and solid lines indicate current positions of the spindle poles and axis, respectively. Bar, 20 μm.