Figure 5.

Pattern of dynein localization during mitosis. NRK cells were fixed and stained for the intermediate chain of dynein (green) and chromosomes (blue). The cells were optically sectioned, and images were deconvolved and reconstructed to show the 90° view of structures on all focal planes. During early prometaphase (A), dynein is localized primarily at the kinetochores of the chromosomes. By late prometaphase (B), the distribution has changed dramatically. Dynein is now visible primarily on spindle poles and astral microtubules. Throughout metaphase and early anaphase (C and D), dynein is predominantly localized in a discontinuous pattern along astral microtubules. Some localization was also observed along interzonal microtubules. Bar, 10 μm.