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. 2003 Mar;71(3):1134–1140. doi: 10.1128/IAI.71.3.1134-1140.2003

FIG. 2.

FIG. 2.

Southern blot analysis of mutants. DNA was extracted from wild-type (WT) and mutant (mut) strains and analyzed. The gels show diagnostic digests that confirm their genotypes. devR, EcoRI/XhoI digest probed with 0.8-kb NotI fragment; trcS, XhoI digest probed with 2.3-kb XhoI fragment; trcXY, EcoRI digest probed with 2.5-kb SmaI fragment; kdpDE, XhoI digest probed with 3.7-kb XhoI fragment; narL, BamHI digest probed with 2.4-kb mutagenesis fragment; Rv3220c, XhoI digest probed with 1-kb mutagenesis fragment.