FIG. 2.
Infection with the high-virulence RA T. cruzi strain impairs DC stimulation of CD4 and CD8 T-cell alloresponse. Irradiated splenic DC (2 × 105/well) from acutely RA- or K98-infected and control C3H/HeNk mice were cocultured with CD4 and CD8 T cells (2 × 105/well) from the lymph nodes of uninfected C57BL/6b (C57) mice. As a control for background proliferation, responder CD4 or CD8 T cells (C57BL/6b) were cocultured in the presence of syngeneic splenic DC (control C57). Cell proliferation was measured on day 4 after a 24-h pulse with [3H]-thymidine (3H-T). Values are the mean of triplicate cultures ± standard deviation. *, P < 0.05; **, P < 0.001 compared to control C57BL/6 stimulator cells.