FIG. 4.
Effect of in vivo T. cruzi infection on ability of DC to activate in vitro T. cruzi-specific T-cell proliferative responses. Irradiated splenic DC (4 × 104/well) from acutely RA- or K98-infected and control C3H/HeNk mice were cocultured with CD4 T cells (5 × 105/well) from the lymph nodes of chronically RA- or K98-infected or control mice at 37°C in the presence of T. cruzi lysate (50 μg/ml) in RPMI 1640-10% FBS medium in 96-well microplates in triplicate in a final volume of 200 μl. Background proliferation of DC alone was ≤400 cpm and of T cells alone was ≤500 cpm. Cell proliferation was measured on day 7 after a 24-h pulse with [3H]thymidine. Values are the means of triplicate cultures ± standard deviation. Results for one of three independent experiments are shown.