TABLE 1.
pmp PCR primers
| Primer | Sequence | Nucelotide locationa and direction |
|---|---|---|
| pmpA1-FOR | 5′-ATG AAT CGA GTT ATA GAA ATC CAT GC | 145, forward |
| pmpA1-REV | 5′-TTA GAA GCC AAC ATA GCC TCC G | 3061, reverse |
| pmpC1-FOR | 5′-GAC TGC AAT GTT AGC AAA TTA GGA TA | 94, forward |
| pmpC1-REV | 5′-CAT ACG AGC ACC GCA GTT CA | 5288, reverse |
| pmpD1-FOR | 5′-CCT CAA GCG GTT TTA TTG TTA GAC C | 181, forward |
| pmpD1-REV | 5′-CAA TCC AGT ATT CGC CTC ATA TCC T | 4590, reverse |
| pmpE1-FOR | 5′-CTT ATG CCC AAC TCA GTT CCA GA | 151, forward |
| pmpE1-REV | 5′-TCG CAG AGC AAT TTC CCC AT | 2942, reverse |
| pmpF1-FOR | 5′-ATG AAA CGG ATA CGC TAC AGT TC | 83, forward |
| pmpF1-REV | 5′-GAC CAG AGC TCC TCC TGC AT | 3086, reverse |
| pmpG1-FOR | 5′-CAA GGA ATT TAC GAT GGG GAG A | 127, forward |
| pmpG1-REV | 5′-TCC TGC ACT CAA ACC ATA ACC | 3031, reverse |
| pmpH N1 | 5′-TCT CCT CAA GTG TTA ACA CCT AAT G | 76, forward |
| pmpH1-REV | 5′-AAA GAT TCT ATT CAA GCC CAT GG | 3035, reverse |
| pmpI1-FOR | 5′-CAG GAT CCC TTA GGT GAA ACC G | 73, forward |
| pmpI1-REV | 5′-CAG GGA GTA ACT ATG GCT TTG CC | 2610, reverse |
a
Primer locations indicate base position of each primer in serovar D for each pmp gene.