TABLE 1.
Strains and plasmids used in this study
| Strain, phage, or plasmid | Descriptiona | Reference or source |
|---|---|---|
| P. aeruginosa | ||
| PA103 | Clinical isolate of wild-type cytotoxic strain | 22 |
| PA103 exsA | PA103 with chromosomal disruption of the exsA locus; Spr Smr | This study |
| PA103 ΔexoU exoT | PA103 with exoU deletion and exoT disruption; Tcr | 35 |
| PA103 dsbA | PA103 with chromosomal disruption of the dsbA locus; Spr Smr | This study |
| PA103 exsA dsbA | PA103 with chromosomal disruption of both exsA and dsbA loci; Spr Smr Gmr | This study |
| PO4 phage | Pillus-specific lytic phage | David Bradley |
| Plasmids | ||
| pCR2.1-TOPO | PCR cloning vector, Apr Kmr | Invitrogen |
| pDN19lacΩ | Broad-host-range plasmid containing a promoterless lacZ gene; Spr Smr Tcr | 33 |
| pEX18Tc | Suicide vector for recombination by site-specific excision; Sucs Tcr | 18 |
| pHW0018 | exoT promoter of PA103 fused to promoterless lacZ in pDN19lacΩ; Spr Smr Tcr | 15 |
| pHW0203 | exsA promoter of PA103 fused to promoterless lacZ in pDN19lacΩ; Spr Smr Tcr | This study |
| pHW0206 | PCR-amplified dsbA gene from PA103 in pCR2.1-TOPO; Apr Kmr | This study |
| pHW0210 | dsbA gene of PA103 cloned in pUCP19; Apr | This study |
| pHW0212 | Disruption of dsbA gene by Gmr cassette in pHW0206; Apr Kmr Gmr | This study |
| pHW0216 | dsbA gene with Gmr cassette insertion cloned in pEX18Tc; Tcr | This study |
| pMSZ5 | PAK pilA promoter fused to a promoterless lacZ in pDN19lacΩ; Spr Smr Tcr | 20 |
| pUCP19 | Broad-host-range shuttle vector, Apr | 30 |
a
Resistance markers: Apr, ampicillin; Kmr, kanamycin; Gmr, gentamicin; Spr, spectinomycin; Smr, streptomycin; Tcr, tetracycline. Sucs, sucrose-sensitive marker encoded by sacB.