Figure 6.

Effects of nocodazole on CNF1-induced cell multinucleation. HEp-2, MDCK, or HeLa cells grown in 24-well plates were washed briefly with HEPES-buffered medium and incubated with nocodazole for 1 h at 4°C. CNF1 (10⁻¹⁰ M) was added for 90 min at 37°C still in the presence of nocodazole. Then the medium was removed, and cells were incubated with fresh medium containing bafilomycin A1 to block further entry of CNF1. Cells were incubated for an additional 24 h in the presence of bafilomycin A1, and multinucleation was recorded and expressed as in Figure 5.