FIG. 2.

Expression of TLR2 and TLR2-induced apoptosis in a primary human Schwann cell line. (A) Primary human Schwann cells isolated from nerve explants showed typical Schwann cell morphology when labeled with a MAb to Schwann cell-specific p75. (B) Primary Schwann cells expressed TLR2 and the antigen-presenting molecule MHC class I (MHC-I). Specific antibodies are indicated by thick lines; isotype control antibodies are indicated by thin lines. (C) The 19-kDa lipopeptide induced apoptosis in Schwann cells in a TLR2-dependent manner. (D) Quantification of apoptotic cells. The nuclear morphology of apoptotic cells was assessed after Hoechst staining and exposure to UV light. For quantitative analysis, percent nuclear fragmentation was calculated based on an examination of at least 100 target cells in multiple vision fields. The data are shown as mean and standard error of the mean. The data are from a single donor, since this experiment was performed to confirm the data from the cell lines.