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Autoradiogram of transcytosed 125I-BoNT/A across Calu-3 cells. 125I-BoNT/A that had passed through Calu-3 cells was separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (10%). Lanes a, c, and e, nonreducing conditions; lanes b, d, and f, reducing conditions; lanes a and b, labeled starting material; lanes c and d, material incubated with medium at 37°C for 18 h; lanes e and f, transcytosed material.
