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. 2003 Mar;71(3):1147–1154. doi: 10.1128/IAI.71.3.1147-1154.2003

FIG. 7.

FIG. 7.

Survival rate and anti-BoNT/A IgG responses after i.n. administration of HC. Mice were immunized i.n. with three different doses of the HC on days 0, 7, 14, 21, and 28. Challenges with 3 × 104 LD50 of BoNT/A were given i.p. on day 35. Serum samples were also taken on day 35, and serum antibody titers were determined by a BoNT/A-specific IgG ELISA. The values represent the means (plus standard deviations) for five mice.