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. 2003 Mar;71(3):1155–1160. doi: 10.1128/IAI.71.3.1155-1160.2003

FIG. 1.

FIG. 1.

Transcription of P42 gene in NIH 3T3 cells. Plasmid pcDNA3/P42 or pcDNA3 was transfected with Lipofectamine into NIH 3T3 cells. Production of specific mRNA was assessed 48 h later. Total RNA was purified from transfected cells and amplified by RT-PCR with P42-specific primers, with β-actin as the control. Lane M, molecular size markers: 1,500 bp, 1,200 bp, and ladders from 1,000 bp to 100 bp. Lane 1, RT-PCR for β-actin gene from NIH 3T3 cells without transfection. Lane 2, RT-PCR for P42 gene from nontransfected NIH 3T3 cells. Lane 3, RT-PCR for β-actin gene from NIH 3T3 transfected with pcDNA3/P42. Lane 4, RT-PCR for P42 gene from NIH 3T3 transfected with pcDNA3/P42.