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Western analysis of serum from mice vaccinated for 14 weeks. Sera from 12 mice injected with pcDNA3/P42 were pooled to perform Western analysis. Lane 1, molecular size markers (from the top: 175 kDa, 83 kDa, 62 kDa, 47.5 kDa, 32.5 kDa, 25 kDa, and 16.5 kDa). Lane 2, purified P42 from E. coli carrying plasmid pGEX4T/P42. Lane 3, total protein of M. hyopneumoniae.
