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Growth inhibition assay of M. hyopneumoniae. The growth rate of the mycoplasmal cells was monitored by measuring the OD560. The growth-inhibitory effects of antiserum from mice immunized with (▴) pcDNA3/P42 (1:100 dilution), (×) pcDNA3/P42 (1:1,000 dilution), or (▪) pcDNA3 (1:100 dilution) and (♦) of no antiserum were tested. The open circle represents the OD560 of the growth medium without cells.
