Figure 1.

Schematic representation of plasmid vectors used for stable transformation of Giardia trophozoites. A RAN-neo cassette conferring constitutive G418 resistance is cloned immediately adjacent to a recombinant CWP1 locus in a head-to-head conformation on a pBluescript backbone. All four domains of CWP1 (I–IV) are sequentially added to the N-terminal side of a GFP reporter gene. In construct pC0-PP2, the CWP1 3′ flanking sequence was replaced by that of the Giardia PP2 gene. CWP1 domains are indicated by roman numerals. Promoters and corresponding direction of transcription are represented by bent arrows.