Dot blot of mortality rate and MTD of chick embryos inoculated with 14 human asymptomatic carriage strains (○), 17 human sporadic strains (shaded triangle), and 3 epidemic-associated strains of L. monocytogenes (▴). For each strain, ca. 1.5 to 2.5 log10 CFU were inoculated into five 14-day-old chick embryos, for which the mortality was monitored daily for 6 days. Groups A, B, and C represent the three levels of virulence obtained from the chick embryos assay.