FIG. 2.

Listerial adhesion to and entry into Caco-2 cells. For adhesion (▪) and entry (□) rates, the number of viable bacteria was determined after 30 min and 3.5 h of infection, respectively, by cell lysis and inoculation onto ACA plates by using appropriate dilutions. Groups A, B, and C represent the three levels of virulence obtained from the chick embryos. The results are expressed as the mean percentage (of initial inoculum) of viable recovered bacteria per well from two experiments analyzed in duplicate. Vertical bars depict the standard deviation.