FIG. 2.

Effect of AG or NMMA on the infection of PMΦ or BMMΦ by C. burnetii phase II bacteria. Cultures were infected for 24 h, washed, and kept in AG- or NMMA-containing medium for the 6 days of the infection. (A) Percentage of cells with LRVs and nitrite production by control and AG- or NMMA-treated PMΦ and BMMΦ cultures. (B) Average of the relative bacterial load per LRV. (C) Fluorimetrically estimated total bacterial load per well in the same exper-iment shown in panels A and B. For clarity, the same nitrite data are shown in panels A, B, and C. Means ± the standard deviations of one representative experiment from three with similar results. Asterisks indicate that differences between control (CTR) and treated cultures (AG or NMMA) were statistically significant (P < 0.01).