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. 2003 Mar;71(3):1225–1233. doi: 10.1128/IAI.71.3.1225-1233.2003

FIG. 3.

FIG. 3.

Effect of SNP on the frequency of LRVs and their bacterial load in C. burnetii phase II-infected BMMΦ. SNP was added to the cultures at the time of infection and was kept in the medium until the end of experiment (6 days). The percentages of cells with LRVs (A) and the relative bacterial loads in the LRVs (B) are shown, together with the production of nitrite, as a function of the SNP concentration. The same nitrite data are shown in panels A and B as the means ± the standard deviations of one representative experiment from three with similar results. Significant differences between untreated control and treated cultures are indicated (✽, P < 0.05; ✽✽, P < 0.01). (C to E) Confocal superimposed images of DAPI fluorescence (black) and Nomarski differential interference contrast (gray). Images of representative cells illustrate the difference in bacterial loads inside the large vacuoles (arrow) of cells that were either left untreated (C) or were treated with 50 μM (D) or 100 μM (E) SNP. N, cell nuclei. Bar, 10 μm.