TABLE 1.
Effect of IFN-γ on the production of NO by control or by C. burnetii phase II-infected BMMφ or PMφ
Host cell | Production of NO (μM nitrite/105 macrophages/24 h ± sd)
|
|||
---|---|---|---|---|
Uninfected
|
Infecteda
|
|||
No addition | With IFN-γ | No addition | With IFN-γ | |
BMMφ | 0 | 0 | 1.8 ± 0.08 | 12.3 ± 0.20 |
PMφ | 1.6 ± 0.04 | 4.1 ± 0.41 | 6.6 ± 0.20 | 16.9 ± 0.40 |
Macrophages were infected with C. burnetii for 24 h, and nitrite was measured in the culture supernatants. NO production was inferred from the nitrite content of culture supernatants. Cells were exposed to IFN-γ or to control medium for the duration of the infection.