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Time-dependent expression of MIP-1α mRNA after i.t. AdMIP-1α administration. Lungs were harvested on days 1, 2, 4, 7, and 14 after i.t. administration of Adcmvplpa (5 × 108 PFU) and AdMIP-1α (5 × 108 PFU), and then MIP-1α and β-actin mRNA expression was determined by RT-PCR (30 and 25 cycles for MIP-1α and β-actin, respectively). Molecular weight markers are shown to the right. Each lane represents two animals per time point; experimental n = 4.
