FIG. 1.

Mobility shift of Rho in lymphoid cells upon treatment with CNF-1. (A) Jurkat T cells (10⁷ cells/assay) treated or not treated for 4 or 24 h with different concentrations of CNF-1 (1, 10, 100, and 300 pM and 1, 1.5, and 3 nM) were lysed. Rho proteins were [³²P]ADP ribosylated for 90 min with C3 exoenzyme. Deamidation of Rho was visualized by SDS-12% PAGE, followed by blotting and autoradiography as described in Materials and Methods. (B) Densitometric scanning of the deamidated Rho protein. (C) Upshift of Rho in T lymphocytes and Jurkat and HEp-2 cells treated or not treated for 4 h with 3 nM of CNF-1 was analyzed by [³²P]ADP ribosylation.