TABLE 2.
Protective efficacy against acute fatal pneumonia due to ExoU+ PAO1 after active immunization with PAO1ΔaroA using different strains of inbred mice and different routes of immunization and challenge
| Mouse strain | Immunization route | Challenge route | Inoculum (CFU) | Multiple of LD50 | No. of surviving mice/no. of mice challenged in vaccine group:
|
|
|---|---|---|---|---|---|---|
| E. coli HB101 | PAO1ΔaroA | |||||
| C3H/HeN | i.n. | i.n. | 5 × 107 | 70 | 0/16 | 16/16b |
| C3H/HeN | i.n. | i.n. | 5 × 108 | 700 | 0/6 | 1/5 |
| BALB/c | i.n. | i.n. | 8 × 108 | 1,100 | 0/15 | 15/15b |
| C57BL/6 | i.n. | i.n. | 5 × 107 | 70 | 0/9 | 9/9c |
| FVB/N | i.n. | i.n. | 5 × 107 | 70 | 0/5 | 5/5c |
| C3H/HeN | i.p. | i.t.a | 5 × 107 | 70 | 0/4 | 4/4c |
| C3H/HeN | i.n. | i.p. | 1 × 108 | 5 | 1/8 | 7/8c |
a
i.t., intratracheal.
b
P < 0.001 (Fisher exact test).
c
P < 0.05 (Fisher exact test).