Figure 5.

Kinetics of antibody-tagged P-selectin and CD63/lamp3 transport to Weibel–Palade bodies. Cells were grown in the presence of monoclonal antibodies against CD63/lamp3 (A) or P-selectin (B). At appropriate time intervals, cells were fixed and analyzed by fluorescence microscopy. Anti-CD63/lamp3 antibodies were used at a concentration of 20 μg/ml in the medium, whereas antibodies against P-selectin conjugated to Alexa 568 were used at a concentration of 5 μg/ml. CD63/lamp3 and vWF were revealed by indirect immunofluorescence. Arrowheads show vWF-positive Weibel–Palade bodies. As in Figure 4, exposure times were adjusted so that labeled structures could be well resolved. Bar, 10 μm.