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. 2003 Mar;71(3):1370–1378. doi: 10.1128/IAI.71.3.1370-1378.2003

FIG. 3.

FIG. 3.

Donor PECs used for reconstitution of B6 NUDE mice. (a to d) Twenty B6+/+ mice were immunized with 25 B. pahangi L3. PECs were collected at 3 weeks following immunization and B cells were purified. Panels a and b show the positively selected fraction. Gates R1, R3, and R4 in panel a demarcate small lymphocytes, macrophages, and eosinophils, respectively. Panels c and d show the B-cell-depleted flowthrough fraction. Panel d is gated on small lymphocytes (R1). Cells were stained with anti-CD19-FITC and B220-PE, and anti-CD3-PE. (e and f) PECs were collected from 35 naïve B6+/+ mice. B lymphocytes were purified using the same protocol and reagents as described for primed donor cells. Both panels show positively selected CD19+ peritoneal cells. Cells were stained with anti-CD19-FITC and B220-PE.