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Proportions of IgE+ and CD5+ B lymphocytes in the peritoneal cavity of nude mice reconstituted with naïve or primed peritoneal B cells. PECs were gated on lymphocytes and analyzed using anti-IgE-FITC and anti-CD19-biotin or anti-CD19-FITC and anti-CD5-PE. Each bar represents the average from 10 (primed B and no treatment [No Tx] groups) or 9 (naïve B group) mice. The values from the primed B group for both cell types were significantly different from the other groups (P < 0.000).
