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Immunoblot assays of preexacerbation and postexacerbation serum samples to detect serum IgG. All sera were tested at a dilution of 1:100. Each lane has a GST fusion peptide containing amino acids 261 to 427 (arrow). The band at ∼80 kDa is an E. coli protein that copurifies with recombinant GST fusion peptides. Molecular masses are noted in kilodaltons on the right.
