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. 2003 Mar;71(3):1527–1537. doi: 10.1128/IAI.71.3.1527-1537.2003

FIG. 5.

FIG. 5.

EtxB(H57S) lacks the immunogenic and adjuvant properties of EtxB in vivo. Groups of eight NIH mice were immunized intranasally under light halothane anesthesia on days 0, 7, and 14 with 10 μg of EtxB, EtxB(G33D), or EtxB(H57A) alone or admixed with 10 μg OVA. Control animals were given PBS. On day 21, animals were bled by cardiac puncture, and EtxB- and OVA-specific serum IgG antibody titers were determined using ELISA as described in Materials and Methods. Titers are given as means + standard deviations (error bars). (A) EtxB-specific serum IgG titers. Differences between groups immunized with EtxB or EtxB(H57S) were statistically significant (*, P = 0.002). (B) OVA-specific serum IgG titers. Differences between groups immunized with EtxB plus OVA or OVA alone were statistically significant (*, P = 0.042).