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. 2003 Mar;71(3):1527–1537. doi: 10.1128/IAI.71.3.1527-1537.2003

FIG. 7.

FIG. 7.

EtxB(H57S) is capable of delivering a peptide into the MHC-I antigen presentation pathway. EtxB(H57S)-mediated delivery of class I peptides as assessed by analysis of IL-2 release by RF33.70 T-cell hybridoma. JAWSII cells were incubated with 19-mer peptide alone or peptide admixed with or conjugated to EtxB, EtxB(G33D), or EtxB(H57S) at the equivalent concentration of 100 nM peptide for 2 h. Cells were then fixed with 1% paraformaldehyde and incubated overnight with RF33.70 T-cell hybridoma, specific for SIINFEKL peptide in the context of MHC-I. The 8-mer peptide SIINFEKL and PBS were used as positive and negative control, respectively. Duplicate samples were tested, and presented data are given as means + SEM (error bars).