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Influence of polymyxin B on the area of a monolayer and the adsorbed calcium. Injection of polymyxin B (350 μM) underneath a LPS F515 monolayer at a constant lateral pressure of 27 mN m-1 led to (A) no change of the area of the monolayer (B) a decrease of the calcium adsorbed on the monolayer. Bathing solution: 12.5 μM Ca2+ doped with radioactive 45Ca2+ resulting in a relative β-activity of 250 Bq/ml, buffered with 5 mM HEPES, and adjusted to pH7.
