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. 2003 Mar;71(3):1109–1115. doi: 10.1128/IAI.71.3.1109-1115.2003

FIG. 3.

FIG. 3.

Regulation of antimicrobial peptide production in response to Salmonella infection evaluated by AU-PAGE and Western blot analysis. Mice were orally inoculated p.o. with buffer alone (lanes 1 and 2) or buffer containing wild-type Salmonella 14028s (lanes 3 and 4) or Salmonella TK93 (lanes 5 and 6). The mice were sacrificed after 24 h. Acetic acid extracts were made from the distal small intestinal epithelium. Then, 50 μg of protein was fractionated by Tricine-PAGE. (A) Western blot analysis of Tricine-PAGE with anti-procryptdin antibody (1:1,000; generously provided by Andre Ouellette, University of California at Irvine, Irvine). P, procryptdin control (0.5 μg). (B) Western blot analysis of Tricine-PAGE with anti-lysozyme antibody (1:500). L, human lysozyme control (1 μg). (C) A total of 25 μg of protein was fractionated by AU-PAGE. The top panel shows the top portion of the gel, which was Coomassie blue stained to demonstrate equal protein loading. The bottom panel shows the lower portion of the silver-stained gel. Cryptdins are the fastest-migrating proteins (bracket).