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. 2003 Mar;71(3):1109–1115. doi: 10.1128/IAI.71.3.1109-1115.2003

FIG. 4.

FIG. 4.

Activation of p38 MAPK pathway by Salmonella serovar Typhimurium evaluated by Western blot analysis. Mice were orally inoculated p.o. with buffer alone (lanes 2 to 4) or with buffer with Salmonella TK93 (lanes 5 to 7) or wild-type Salmonella 14028s (lanes 8 to 10). The mice were sacrificed after 24 h. Lysates were made from the distal small intestinal epithelium and fractionated by SDS-PAGE. Western blots were analyzed with phospho-p38 (A) and p38 (B) antibodies at 1:1,000. Each lane represents one mouse. Arrow, phosphorylated, active p38. The first lane of the phospho-p38 blot (+) contains lysate from NIH 3T3 cells that had been treated with sorbitol, which activates the p38 MAPK pathway.