FIG. 5.

Inhibition of p38 MAPK pathway by SB 203580 after Salmonella infection evaluated by MAPKAPK2 assay. Mice were orally inoculated p.o. with either wild-type Salmonella (12 mice) in buffer or with buffer alone (12 mice). At 24 h postinoculation, half of each group was given a 25-mg/kg oral dose of SB 203580 in acidified tragacanth (3). The other half was given vehicle alone. The mice were sacrificed 2 h thereafter. Lysates were prepared from distal small intestinal epithelium. MAPKAPK2 was immunoprecipitated from these lysates and subjected to in vitro kinase assays. Results show averages of six mice per group (Student t test: P < 0.005 compared p.o. buffer group to Salmonella group, P < 0.0005 comparing Salmonella plus SB203580 to Salmonella plus vehicle, and P < 0.05 comparing p.o. buffer plus SB203580 to p.o. buffer plus vehicle.).