TABLE 2.

Effects of p38 inhibition on modulation of antimicrobial peptide expression by Salmonella

mRNA	Mean mRNA level (cpm) ± SE or I/U ratio with:
	No inhibitor			Inhibitor (SB 203580)
	U	I	Ratio	U	I	Ratio
Cryptdin	4.5 ± 0.6	2.5 ± 0.3	0.55b	3.7 ± 0.4	3.4 ± 0.7	0.92
Lysozyme	6.5 ± 0.5	3.3 ± 0.7	0.51b	7.1 ± 0.5	4.9 ± 1.4	0.69
Matrilysin	0.60 ± 0.03	0.58 ± 0.04	0.97	0.61 ± 0.02	0.73 ± 0.09	1.20

^aMice were orally inoculated p.o. with either wild-type Salmonella in buffer or buffer alone. After 24 h, half of each group was given a 25-mg/kg oral dose of SB 203580 in acidified tragacanth. The other half was given vehicle alone. The mice were sacrificed after 2 h. Total RNA was isolated from distal small intestine, fractionated, blotted on to a nylon filter, and hybridized sequentially to G3PDH, cryptdin 1, lysozyme, and matrilysin oligonucleotide probes. The radioactive oligonucleotide signals from the Northern blot analyses were quantitated by using a phosphorimager. The tabulated results show the normalized cpm values for mRNA levels in Salmonella-infected mice (I) and uninfected mice (U) with the calculated standard errors. The tabulated results are representative of two individual experiments.

^bP ≤ 0.005 (Student t test) comparing uninfected and infected mice. Values for both groups are normalized cpm values for mRNA levels (n = four mice per group).