Table 4.

Oligonucleotides used for IRS-PCR and plasmid-specific PCR.

Designation	Sequence	Used for	PCR conditions (°C/seconds)			No. of PCR cycles	Source
			Denaturation	Annealing	Extension
Ps1	5'-GACTCGACTCGCATGCA-3'	Adapter 1, primer IRS-PCR	94/30	55/60 then 60/30	72/90	5 then 30	30
AH2	5'-TGCGAGT-3'	Adapter 1					30
Sal1	5'-PO4-TCGATACTGGCAGACTCT-3'	Adapter 2					30
AX2	5'-GCCAGTA-3'	Adapter 2					30
PsalA	5'-AGAGTCTGCCAGTATCGACA-3'	Primer IRS-PCR	94/30	55/60 then 60/30	72/90	5 then 30	30
PsalC	5'-AGAGTCTGCCAGTATCGACC-3'	Primer IRS-PCR	94/30	55/60 then 60/30	72/90	5 then 30	30
PsalG	5'-AGAGTCTGCCAGTATCGACG-3'	Primer IRS-PCR	94/30	55/60 then 60/30	72/90	5 then 30	30
PsalT	5'-AGAGTCTGCCAGTATCGACT-3'	Primer IRS-PCR	94/30	55/60 then 60/30	72/90	5 then 30	30
CB5	5'-ATAATGAGATTAGAACAACCAAGA-3'	Primer QpH1 PCR	94/120	53/60	72/120	35	56
CB6	5'-TCTTTCTTGTTCATTTTCTGAGTC-3'	Primer QpH1 PCR	94/120	53/60	72/120	35	56
QpRS1	5'-CTCGTACCCAAAGACTATGAATATATCC-3'	Primer QpRS PCR	94/60	54/60	72/120	36	56
QpRS2	5'-AACACCGATCAATGCGACTAGCCC-3'	Primer QpRS PCR	94/60	54/60	72/120	36	56