Skip to main content
. 2006 Jul 5;34(11):3399–3407. doi: 10.1093/nar/gkl432

Figure 2.

Figure 2

Transient transfection of a HA-TFIIIC110 expression vector has little or no effect on VA1 transcript levels in HEK293 cells. (A) Western blot of HEK293 cells transfected with pcDNA3HA-TFIIIC110 and/or pcDNA3HA to a total concentration of 2 μg. Upper panel shows a blot for HA-TFIIIC110, lower panel shows a blot for actin. (B) Primer extension analysis of VA1 and GFP RNA in HEK293 cells transfected with 0.25 μg of each of the VA1 and GFP plasmids and with pcDNA3HA-TFIIIC110 and/or pcDNA3HA to a total concentration of 2 μg. (C) Quantitation of the data from (B) and three identical experiments.