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. 2006 Jul 5;34(11):3399–3407. doi: 10.1093/nar/gkl432

Figure 8.

Figure 8

Induction of HA-Brf1 can stimulate expression of tRNA and 5S rRNA genes in stably transfected HeLa cells. (A) Western blot of HeLa cell clones stably transfected with pTRE2hygHA-Brf1 and grown in the presence or absence of doxycycline for 48 h. The upper panel shows a blot for HA and the lower panel shows a blot for actin. (B) RT–PCR analysis of 5S rRNA, tRNA and ARPP P0 mRNA, as indicated, in HeLa cells stably transfected with pTRE2hygHA-Brf1 and grown in 10% serum in the presence or absence of doxycycline for 48 h. (C) Quantitation of the data shown in (B).