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Sensitivity of MB-PCR. (A) MB-PCRs for the ICSBP promoter from mixtures of DNA from a healthy donor (N, unmethylated) and DNA from the cell line KG-1 (methylated). The relative contribution of each DNA is indicated in the table on the right. (B) DNA samples from three cell lines were subjected to MB-PCR using 10 ng and down to 160 pg of DNA for the M-reaction. The number of amplification cycles (given in parentheses) was adjusted to the amount of DNA.
