Figure 5.

Alcohol-Induced jekyll Expression in Transformed Tobacco Roots.

(A) and (B) Spatial patterns of promoter activity after induction with ethanol in roots of tobacco transformed with pAlc-GUS grown under hydroponics. GUS staining in the epidermis ([A]; arrows) and root hairs ([B]; arrows) as primary sites of promoter activity. Bars = 40 μm.

(C) Disintegration of the outer cell layers in roots of transgenics after the ethanol induction coincides spatially with JEKYLL immunodetected with specific antibodies (shown in inset). Extensive cell damage within two or three outer cell layers of the root are indicated in red. Bar = 150 μm.

(D) to (G) Root hairs of wild-type plants ([D] and [F]) and severe reduction of root hair growth in transgenics expressing JEKYLL ([E] and [G]). Bars = 1 mm.