Figure 9.

Effect of Jekyll Repression on Starch Content and Starch Distribution during Seed Development.

(A) Starch content in developing caryopses 0, 4, and 12 DAF. Error bars indicate sd. FW, fresh weight.

(B) Hybridization of total RNA from wild-type and transgenic caryopses 4, 8, and 12 DAF with the endosperm-specific small subunit of ADP-glucose pyrophosphorylase probe.

(C) to (E) Iodine staining of cross sections of wild-type seeds. Transient starch accumulation is visible in the nucellus 2 DAF (C) and pericarp 4 DAF (D), followed by starch accumulation in endosperm at 8 DAF (E). Note the absence of starch in the nucellar projection.

(F) to (H) Similar staining of caryopses of transgenic N91 shows starch deposition within the nucellar projection at 4 DAF (F). The area of starch deposition increases with time, caryopsis at 6 DAF (G). At 12 DAF, starch is deposited primarily in the pericarp of the transgenic caryopsis (H) but not in the endosperm. Abbreviations are as in Figure 3. Bars = 300 μm.