Table 1.
Phenotypic Characteristics of Selected Lines of T2 Transgenic Barley Plants Transformed with the Construct pVECNpass
| Plant Line | Level of jekyll Expression (%)a | Plant Height (cm) | Number of Spikes per Plant | Number of Corns per Spike | 100 Corn Weight (g) | Starch Content (mg/g) |
|---|---|---|---|---|---|---|
| Wild type | 100.0 | 56.4 ± 2.3a | 18.0 ± 2.4a | 21.6 ± 3.8a | 4.42 ± 0.67a | 455 ± 46a |
| N18 | 74.8 | 52.0 ± 2.0a | 17.7 ± 2.4a | 20.2 ± 4.9a | 3.04 ± 0.05b | 365 ± 26b |
| N61 | 21.3 | 49.3 ± 3.3b | 12.8 ± 4.8a | 13.4 ± 2.8b | 2.10 ± 0.36c | 340 ± 18b |
| N81 | 20.8 | 52.4 ± 3.2a | 15.0 ± 4.0a | 6.8 ± 4.6c | 2.14 ± 0.23c | ND |
| N91 | 25.3 | 51.4 ± 7.7a | 15.2 ± 5.0a | 5.2 ± 3.3c | 2.02 ± 0.38c | 361 ± 63b |
The values are given as means ± sd. Values followed by the same letter within a column are not significantly different at P < 0.05. ND, not detected.
a
Levels of jekyll mRNA expression were estimated by RNA gel blot analysis at 4 DAF. Hybridization signals were quantified as described (Weschke et al., 2000) and given in relative units as a percentage of expression to the wild type estimated as 100%.