Figure 3.

Map-Based Cloning of the rol1 Locus.

(A) rol1-1 and rol1-2 were independently mapped on chromosome 1, south of nga111, to an interval of 100 and 38 kb, respectively, on BAC T30F21 using self-made uzu (cleaved-amplified polymorphic sequence [CAPS] and simple sequence length polymorphism [SSLP]) markers.

(B) The RHM1 gene (At1g78570) encodes a 2673-nucleotide transcript encoding a protein of 668 amino acids. Sequencing revealed point mutations at positions corresponding to the N-terminal dehydratase domain. rol1-1 is a nonsense mutation at position 318, and rol1-2 is a missense mutation changing an Arg at position 283 to a Lys. Numbers indicate nucleotide (above) or amino acid positions (below). Black boxes indicate the three exons of the RHM1 gene. Striped box, dehydratase domain of RHM1; shaded box, epimerase/reductase domain of RHM1.