Figure 6.

rol1 Mutants Are Affected in RG I.

Wild-type, lrx1, lrx1 rol1-1, and lrx1 rol1-2 plants were embedded and transverse sections of roots stained with the LM5 antibody, detecting galactan side chains of RG I. A strong reduction in staining (green fluorescence) in the lrx1 rol1-1 and lrx1 rol1-2 mutants compared with the wild type or lrx1 is observed. The sections are not of tissue of the identical developmental stage. Sections of lrx1 and lrx1 rol1-2 are of younger root tissue than those of the wild type and lrx1 rol1-1, as can be deduced from the degree of vacuolation of the trichoblasts. As labeling with LM5 is not found in epidermal cells, and labeling in the wild type and lrx1 is comparable; this difference is not relevant. Arrows indicate two root hair forming trichoblasts located over anticlinal walls of two cortex cells. EP, epidermis; C, cortex; EN, endodermis; S, stele. Bar = 100 μm.