Expression of EBOV-GP mutants. (A) Schematic representation of the EBOV-GP mutants analyzed. SP, signal peptide; FP, fusion peptide; TM, transmembrane domain. (B) The indicated EBOV-GP variants were transiently expressed in 293T cells, and GP expression in cellular lysates was analyzed by Western blotting. Rabbit serum raised against ZEBOV-GP was used for detection. Similar results were obtained in two independent experiments. EBOV-GP variants were transiently expressed in 293T cells, and GP expression in cellular lysates was analyzed by Western blotting. Rabbit serum raised against ZEBOV-GP was used for detection. Similar results were obtained in two independent experiments.