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Effect of UL25 concentration on the amount bound to UL25-negative capsids. UL25 synthesized in insect cells was added to KUL25NS capsids and incubated as described in Materials and Methods. Capsids were then isolated by sucrose gradient centrifugation, and the amount of UL25 present was determined by immunoblotting. The amount of UL25 present in wild-type (wt) 17MP A capsids was estimated from capsids isolated in a companion sucrose gradient and analyzed on the same blot.
