TABLE 3.
Immunoelectron-microscopic localization of UL25: location of specifically bound gold beadsa
| Bead location | No of beads (%) with the indicated location in:
|
|
|---|---|---|
| C capsidsb | B capsids | |
| Vertex | 184 (71) | 53 (64) |
| Nonvertex | 76 (29) | 30 (36) |
| Total | 260 (100) | 83 (100) |
a
Capsids were labeled with anti-UL25 polyclonal antibody followed by gold-labeled secondary antibody as described in Materials and Methods. Beads were assigned a vertex or nonvertex location based on analysis of electron micrographs (such as Fig. 2) of stained capsids.
b
Capsids were prepared from the nuclei of 17MP-infected BHK-21 cells as described in Materials and Methods.